代写论文：一种新的抗细菌大肠杆菌素的发现

代写论文：一种新的抗细菌大肠杆菌素的发现

在第二阶段的实验水平较低的细胞治疗丝裂霉素C和伴随免疫印迹支出抗体培育对大肠杆菌素Ia兔子。巷1标记,巷2纯化蛋白,巷3 1 h感应,巷4 2 h,lane5 3 h,巷5 5 h。

大肠杆菌素X基因放大通过使用创建另外两个引物的PCR基因称为Ncol和Xhol。PCR必须通过克隆的片段pET28avector主要提供与他的标签说明没有抑制糖基孔隙形成毒素活动(布劳恩等人1994)。此外,在色谱系统中,这种蛋白质由步骤后的协议。在列加载缓冲40毫升混合物含磷酸钠氯化钠和咪唑(50毫米,300毫米和10毫米),颗粒被停职了。这种混合蛋白酶抑制剂DNase和核糖核酸酶被添加在声波降解法。又添加了所有这些元素的混合,冰的孵化提取的DNA和RNA的45分钟为确保污染被消除。这些颗粒状细胞内通过离心的过程在4摄氏度JA20使用增加了过滤和蛋白酶的抑制剂补充道。

代写论文：一种新的抗细菌大肠杆菌素的发现

In the second phase of the experiment the cells were treated with lower levels of mitomycin C and an accompanying western blot spending antibodies nurtured in rabbits against colicin Ia. Lane 1 markers, lane 2 purified protein, lane 3 1h induction, lane 4 2h, lane5 3h, lane 5 5h.

The colicin X gene was amplified through the use of primers by PCR for creating two other genes known as Ncol and Xhol. The fragment of PCR had to go through cloning with a pET28avector leading towards providing the C-terminus with a His tag illustrating no inhibition of pore forming toxins activity (Braun et al 1994). Further, on a chromatography system, this protein was run by following the steps of protocol. In column loading buffer with 40 ml mixture inclusive of sodium phosphate, sodium chloride and Imidazole (50 MM, 300 MM and 10 MM respectively), the pellet was suspended again. To this mixture inhibitors of protease with DNase and RNase were added before sonication.  The mixture of all these elements were added again and the incubation of ice extraction over a period of 45 minutes for ensuring contamination of DNA and RNA were eliminated. Those pelleted cells through the process of centrifugation within JA20 at 4 degree Celsius were uses to which a filter was added and the inhibitors of protease were added.